Malondialdehyde (MDA) Assay Kit: 200 Tests

NWLSS | Northwest Life Science Specialties, LLC.
Malondialdehyde (MDA) Assay
NWK-MDA01 $395.00 each

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A colormetric assay kit for detection of Malondialdehyde (MDA) or Thiobarbituric Acid Reactive Substances (TBARS) in multiple species and sample types. Designed for simplicity and affordability, this assay utilizes various improvements to provide the most dependable data among commercial assays of this type.

Advantages of the NWLSS™ Malondialdehyde Assay:
  • BHT and EDTA are added to the sample and reaction mixture to minimize artifact oxidation.
  • Reduced reaction temperature minimizes the decomposition of lipid hydroperoxides.
  • Optimized reaction pH facilitates hydrolysis of MDA-protein adducts for better recovery of malondialdehyde (MDA).
  • Cleaner output through optimized data reduction using dual wavelength or 3rd derivative analysis of spectroscopic scan data.

Introduction

Lipid peroxidation has been established as a major mechanism of cellular injury in many biological systems of plant and animal origin. The mechanism involves a process whereby unsaturated lipids are oxidized to form additional radical species as well as toxic by-products that can be harmful to the host system. Polyunsaturated lipids are especially susceptible to this type of damage when in an oxidizing environment and they can react to form lipid peroxides.

Lipid peroxides are themselves unstable, and undergo aditional decomposition to form a complex series of compounds including reactive carbonyl compounds. Polyunsaturated fatty acid peroxides further react to form malonaldehyde (MDA).

MDA can be found in most biological samples including foodstuffs, serum, plasma, tissues and urine, as a result of lipid peroxidation, and has become one of the most widely reported analytes for the purpose of estimating oxidative stress effects on lipids.

Test Principle

This assay is based on the reaction of malondialdehyde (MDA) with thiobarbituric acid (TBA); forming a MDA-TBA2 adduct that absorbs strongly at 532 nm.

Thiobarbituric acid reactive substances (TBARS) Reaction
MDA - TBA Reaction

This reaction is the most popular method for estimating MDA in biological samples. However, interference can be a significant problem in some biological samples if not dealt with appropriately. Our assay provides the most accurate and efficient means of dealing with elevated backgrounds commonly associated with the TBARS reaction.

Submaximal exercise training, more than dietary selenium supplementation, improves antioxidant status and ameliorates exercise-induced oxidative damage to skeletal muscle in young equine athletes
Yong Zhi Foo, Leigh W. Simmons, Gillian Rhodes
Journal of Animal Science ,  Vol. 95 No. 2, p. 657-670

View Abstract

Time-Dependent Toxicity Responses in Daphnia magna Exposed to CuO and ZnO Nanoparticles
Soyoun KimPalas SamantaJisu YooWoo-Keun KimJinho Jung
Bulletin of Environmental Contamination and Toxicology ,  April 2017, Volume 98, Issue 4, pp 502–507

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Chronic Unpredictable Stress Induces Renal Damage In Rats By Oxidative Stress Provoked Apoptosis and Altering the Function of Na+/K+-ATPase
I Bin-Jaliah
Pak J Physiol ,  2016;12(3)

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Dose-dependent effects of cisplatin on the severity of testicular injury in Sprague Dawley rats: reactive oxygen species and endoplasmic reticulum stress
Kiran Kumar Soni, Hye Kyung Kim, Bo Ram Choi, Keshab Kumar Karna, Jae Hyung You, Jai Seong Cha, Yu Seob Shin, Sung Won Lee, Chul Young Kim, and Jong Kwan Park
Drug Design, Development and Therapy ,  2016; 10: 3959–3968

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Sudan III Azo Dye: Oxidative Stress with Possible Geno and Hepatotoxic Effects in Male Rats
Dildar Konukoglu, Sinem Fırtına, Gokhan Erkol and I. Murat Bolayırlı
International Journal of Science and Research (IJSR) ,  Volume 5 Issue 10, October 2016

View Abstract

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For Research Use Only

Catalog Number: NWK-MDA01
Format: TBA Based Colorimetric
Sample Requirements: Tissue homogenates, cell lysates and plasma
Specificity: Primary specificity is for malondialdehyde (MDA) when using advanced data reduction and/or back extraction techniques. Basic Thiobarbituric acid reactive substances (TBARS) are detected when making single wavelength 532nm measurements.
Sensitivity: 0.1 µM MDA in the sample
Standard Range: 1.0 - 4.0 mM
Storage and Stability: 12 months from date of manufacture when stored at 2-8 C
Kit Contents: 5 X TBA in powder form
5 X H3PO4; ready to use
5 X TMOP Calibrators; ready to use
1 x Butylated Hydroxytoluene (BHT; ready to use)
1 x Assay Buffer
Random Citation | NWK-MDA01

Chronic nicotine exposure exacerbates acute renal ischemic injury 
Istvan Arany, Samira Grifoni, Jeb S Clark, Eva Csongradi, Christine Maric, and Luis A. Juncos 
American Journal of Physiology - Renal Physiology April 14, 2011, Published Online Ahead of Print

Link to Abstract

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