9(±)-hydroxyoctadecadienoic acid (9± HODE) ELISA Kit

NWLSS | Northwest Life Science Specialties, LLC.
9(±)-hydroxyoctadecadienoic acid (9± HODE) ELISA Kit
NWK-9HOD01 $495.00 each

For questions & ordering, call:
888-449-3091
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The NWLSS™ 9-HODE kit is intended for the quantification of total 9-HODE, which includes both 9(S)-HODE and 9(R)-HODE products of lipid peroxidation in biological samples from any species.

Advantages of the NWLSS™ 9-HODE ELISA:
  • Detects 9-HODE concentrations as low as 100 pg/mL
  • Universally detects samples from any species
  • Provides another useful marker of lipid peroxidation, especially when investigating oxidation of polyunsaturted fatty acids (PUFAS)

Introduction

Linoleic acid, the predominant polyunsaturated fatty acid (PUFA) in the human diet, can be metabolized by cyclooxygenase, lipoxygenase and P450 enzymes. The hydroxyoctadecadienoic acid (HODE) derivatives of linoleic acid, 9(R)-HODE, 9(S)-HODE and 13(S)-HODE, are the most widely distributed of the known linoleic acid metabolites. These compounds exhibit interesting biologic activities, including the regulation of platelet function, maintenance of vascular thromboresistance and transduction of the cellular responses to certain growth factors. HODE derivatives may also influence certain pathological states including, psoriasis, the development of atherosclerosis and the development of cancer.

Test Principle

The NWLSS™ 9-HODE kit is a competitive enzyme-linked immuno-assay (ELISA). Briefly, the 9-HODE present in the samples or standards competes with 9(±)-HODE conjugated to horseradish peroxidase [9(±)-HODE-HRP] for binding to an antibody specific for 9(±)-HODE that is precoated on a microplate. The peroxidase activity of 9(±)-HODE-HRP results in color development when a substrate is added. The intensity of the color is proportional to the amount of 9(±)-HODE-HRP bound and is inversely proportional to the amount of unconjugated 9-HODE present in the samples or standards.

For Research Use Only

Catalog Number: NWK-9HOD01
Format: 96 well competitive ELISA
Sample Requirements: Plasma, tissue homogenates and cell lystates from any species.
Sensitivity: 0.1 ng/mL
Intended Use: Quantitative determination of 9(±)-hydroxyoctadecadienoic acid (9± HODE) in biological samples from any species.
Storage and Stability: 9 months from date of manufacture
Kit Contents: Microwells precoated with anti-9(±)-HODE: 1 X 96 wells
9(±)-HODE Standard (50 mg/mL in ethanol) 1 X 20 mL
Dilution Buffer (5X) 1 X 25 mL
5X Wash Buffer: 1 X 50 mL
9(±)-HODE-HRP Conjugate (40X): 1 X 320 mL
Reagent Trough: 2 Each

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