8-Isoprostane (15-Isoprostane F2t) ELISA Kit for Plasma & Tissue

NWLSS | Northwest Life Science Specialties, LLC.
8-Isoprostane  (15-Isoprostane F2t) ELISA Kit for Plasma & Tissue
NWK-ISO01 $345.00 each

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The 8-Isoprostane (15-Isoprostane F2t) ELISA is intended for the quantification of 8-Isoprostane (15-isoprostane F2t), also sometimes referred to as 8-epi-PGF2a and 8-iso-PGF2a. This ELISA style assay can be used to test serum and tissue samples following solid phase extraction of the isoprostane-containing fraction.

Advantages of the NWLSS™ 8-Isoprostane (15-Isoprostane F2t) ELISA:
  • Detect as little as 50 pg /mL
  • Highly specific for 8-Isoprostane (15-Isoprostane F2t): low or no crossreactivity detected for 9α,11β-Prostaglandin F2α, Prostaglandin F2α, 6-Keto-Prostaglandin F1α, Prostaglandin E2, Prostaglandin D2 or Arachidonic Acid
  • Also provides for the quantification of total 8-Isoprostane (15-isoprostane F2t) following hydrolysis of phospholipids

Introduction

Isoprostanes are prostaglandin-like compounds that are produced by free radical mediated peroxidation of lipoproteins. Levels of 8-Isoprostane (15-isoprostane F2t), a representative isoprostane in biological fluids, have been shown to be useful for assessment of oxidant stress in vivo. 8-Isoprostane (15-isoprostane F2t) has also been shown to be a potent vasoconstrictor in rat kidneys and rabbit lungs and may play a causative role in atherogenesis. Elevated 8-isoprostane (15-isoprostane F2t) levels are associated with hepatorenal syndrome, rheumatoid arthritis, athero-sclerosis, and carcinogenesis.

Test Principle

This assay utilizes a competitive enzyme-linked immunoassay (ELISA) format. Briefly, 8-Isoprostane (15-isoprostane F2t) in the samples or standards is allowed to compete with 8-Isoprostane (15-isoprostane F2t) conjugated to horseradish peroxidase (HRP) for binding to a polyclonal antibody specific for 8-Isoprostane (15-isoprostane F2t) coated on a microplate. Subsequent TMB substrate addition results in a blue color development that is inversely proportional to the quantity of 8-Isoprostane (15-isoprostane F2t) in the original samples or standards. Addition of an acid stop solution causes a color change to yellow where absorbance is read at 450 nM.

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For Research Use Only

Catalog Number: NWK-ISO01
Format: 96 Well Competitive ELISA
Sample Requirements: Urine, serum or tissue samples from any species
Specificity: (100.0%) 8-Isoprostane (15-isoprostane F2t)
(4.1%) 9α,11β-Prostaglandin F2α
(3.0%) 13,14-Dihydro-15-Keto-PGF2α
(<0.01%) 9α,11β-Prostaglandin F2α
(<0.01%) Prostaglandin F2α
(<0.01%) 6-Keto-Prostaglandin F1α
(<0.01%) Prostaglandin E2
(<0.01%) Prostaglandin D2
(<0.01%) Arachidonic Acid
Sensitivity: Lower Limit of Detection (LLD)= 0.005 pg/mL
Effective Range: .05 ng/mL - 10 ng/mL Note that although the analyte is detectable at levels below 50 pg/mL, data within the effective range of this assay will yield greater reproducibility. Investigators may wish to repeat samples with data below this range for added confidence.
Storage and Stability: 9 months from the date of manufacture
Kit Contents: 96-well microtiter plate, pre-coated with 8-Isoprostane (15-Isoprostane F2t) antibody
8-Isoprostane (15-isoprostane F2t) Standard
5X Wash Buffer
5X Dilution Buffer
TMB Substrate
8-Isoprostane (15-isoprostane F2t) HRP Conjugate
Reagent Trough for multi-channel pipettor
Random Citation | NWK-ISO01
Biomarkers of lipid peroxidation related to hypertension in aging
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Hypertension Research,  14 January 2016

View Abstract

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