Urinary 8-Isoprostane (15-Isoprostane F2t) ELISA Kit

NWLSS | Northwest Life Science Specialties, LLC.
Urinary 8-Isoprostane (15-Isoprostane F2t) ELISA Kit
NWK-ISO02 $495.00 each

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This ELISA is intended for use in quantifying free 8-Isoprostane (15-isoprostane F2t), the best characterized isoprostane, in urine samples.

Detailed procedures for extracting 8-Isoprostan (15-Isoprostane F2t) from plasma, cell and tisssue homogenates are included with the NWLSS 8-Isoprostane [NWK-ISO01] kit.

Advantages of the NWLSS™ Urinary 8-Isoprostane (15-Isoprostane F2t) ELISA:
  • Detect as little as 50 pg /mL
  • Highly specific for 8-Isoprostane (15-Isoprostane F2t): low or no crossreactivity detected for 9α,11β-Prostaglandin F2α, Prostaglandin F2α, 6-Keto-Prostaglandin F1α, Prostaglandin E2, Prostaglandin D2 or Arachidonic Acid
  • Allows for simple, quick quantification of 8-Isoprostane (15-Isoprostane F2t) in urine samples from any species without the need for difficult and time consuming extractions


Isoprostanes are prostaglandin-like compounds that are produced by free radical mediated peroxidation of lipoproteins. This kit is for the quantification of 8-Isoprostane (15-isoprostane F2t), also known as 8-epi-PGF2aor 8-iso-PGF2a in urine samples. Levels of 8-Isoprostane (15-isoprostane F2t) in urine are useful for the non-invasive assessment of oxidant stress in vivo. 8-Isoprostane (15-isoprostane F2t) has also been shown to be a potent vasoconstrictor in rat kidneys and rabbit lungs, and may play a causative role in atherogenesis. Elevated isoprostane levels are associated with hepatorenal syndrome, rheumatoid arthritis, atherosclerosis, and carcinogenesis. This kit can be used for the quantification of free 8-Isoprostane (15-isoprostane F2t) in urine serum samples without the need for prior purification or extraction.

Test Principle

This assay utilizes a competitive enzyme-linked immunoassay (ELISA) strategy. Prior to assaying, all samples must be treated with an Enhancing Reagent. After treatment with Enhancing Reagent, 8-Isoprostane (15-isoprostane F2t) in the samples or standards is allowed to compete with 8-Isoprostane (15-isoprostane F2t) conjugated to horseradish peroxidase (HRP) for binding to a polyclonal antibody specific for 8-Isoprostane (15-isoprostane F2t) coated on a microplate. Later TMB substrate addition results in a blue color development that is inversely proportional to the quantity of 8-Isoprostane (15-isoprostane F2t) in the original samples or standards. Addition of an acid stop solution causes a color change to yellow where absorbance is read at 450 nM.

Chitin-glucan fiber effects on oxidized low-density lipoprotein: a randomized controlled trial
HE Bays, JL Evans, KC Maki, M Evans, V Maquet, R Cooper and JW Anderson
European Journal of Clinical Nutrition ,  2013 Jan;67(1):2-7

View Abstract

A randomized, double-blind, placebo-controlled, pilot study to evaluate the effect of whole grape extract on antioxidant status and lipid profile
Malkanthi Evans, Dale Wilson, Najla Guthrie
Journal of Functional Foods March 2014; Volume 7, Pages 680–691

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The relationship between work ability and oxidative stress in Japanese workers
Masanori Ohtaa, Masaharu Kumashiro, Yasumasa Eguchi, Yusaku Morita, Yoshimasa Konno & Hiroshi Yamato
Ergonomics 2014; Volume 57, Issue 8, Pages 1265-1273

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Association of altered hemorheology with oxidative stress and inflammation in metabolic syndrome
Prajwal Gyawali; Ross S. Richards
Redox Report May 2015; Volume 20, Issue 3, pp. 139-144

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Association of abnormal erythrocyte morphology with oxidative stress and inflammation in metabolic syndrome
P Gyawali, RS Richards, PT Bwititi, EU Nwose
Blood Cells, Molecules, and Diseases April 2015; Volume 54, Issue 4, Pages 360–363

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For Research Use Only

Catalog Number: NWK-ISO02
Format: 96 Well Competitive ELISA
Sample Requirements: Urine from any species
Specificity: (100.0%) 8-Isoprostane (15-isoprostane F2t)
(4.1%) 9α,11β-Prostaglandin F2α
(3.0%) 13,14-Dihydro-15-Keto-PGF2α
(<0.01%) 9α,11β-Prostaglandin F2α
(<0.01%) Prostaglandin F2α
(<0.01%) 6-Keto-Prostaglandin F1α
(<0.01%) Prostaglandin E2
(<0.01%) Prostaglandin D2
(<0.01%) Arachidonic Acid
Sensitivity: 0.05 ng/mL
Effective Range: .05 ng/mL - 10 ng/mL
Storage and Stability: 6 months from the date of manufacture
Kit Contents: 96-well microtiter plate, precoated with Anti 8-Isoprostane (15-isoprostane F2t)
8-Isoprostane (15-isoprostane F2t) Standard
Enhancer Reagent
5X Wash Buffer
5X Dilution Buffer
TMB Substrate
8-Isoprostane (15-isoprostane F2t) HRP Conjugate
Reagent Trough for multi-channel pipettor
Random Citation | NWK-ISO02

Oxidative stress biomarkers responses to physical overtraining: Implications for diagnosis 
Konstantinos Margonis, Ioannis G. Fatouros, Athanasios Z. Jamurtas, Michalis G. Nikolaidis, Ioannis Douroudos, Athanasios Chatzinikolaou, Asimina Mitrakou, George Mastorakos, Ioannis Papassotiriou, Kiriakos Taxildaris and Dimitrios Kouretas
Free Radical Biology and Medicine September 2007, Volume 43, Issue 6, Pages 901-910 

Link to Abstract

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