Urinary 8-Isoprostane (15-Isoprostane F2t) ELISA Kit

NWLSS | Northwest Life Science Specialties, LLC.
Urinary 8-Isoprostane (15-Isoprostane F2t) ELISA Kit
NWK-ISO02 $345.00 each

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This ELISA is intended for use in quantifying free 8-Isoprostane (15-isoprostane F2t), the best characterized isoprostane, in urine samples.

Detailed procedures for extracting 8-Isoprostan (15-Isoprostane F2t) from plasma, cell and tisssue homogenates are included with the NWLSS 8-Isoprostane [NWK-ISO01] kit.

Advantages of the NWLSS™ Urinary 8-Isoprostane (15-Isoprostane F2t) ELISA:
  • Detect as little as 50 pg /mL
  • Highly specific for 8-Isoprostane (15-Isoprostane F2t): low or no crossreactivity detected for 9α,11β-Prostaglandin F2α, Prostaglandin F2α, 6-Keto-Prostaglandin F1α, Prostaglandin E2, Prostaglandin D2 or Arachidonic Acid
  • Allows for simple, quick quantification of 8-Isoprostane (15-Isoprostane F2t) in urine samples from any species without the need for difficult and time consuming extractions

Introduction

Isoprostanes are prostaglandin-like compounds that are produced by free radical mediated peroxidation of lipoproteins. This kit is for the quantification of 8-Isoprostane (15-isoprostane F2t), also known as 8-epi-PGF2aor 8-iso-PGF2a in urine samples. Levels of 8-Isoprostane (15-isoprostane F2t) in urine are useful for the non-invasive assessment of oxidant stress in vivo. 8-Isoprostane (15-isoprostane F2t) has also been shown to be a potent vasoconstrictor in rat kidneys and rabbit lungs, and may play a causative role in atherogenesis. Elevated isoprostane levels are associated with hepatorenal syndrome, rheumatoid arthritis, atherosclerosis, and carcinogenesis. This kit can be used for the quantification of free 8-Isoprostane (15-isoprostane F2t) in urine serum samples without the need for prior purification or extraction.

Test Principle

This assay utilizes a competitive enzyme-linked immunoassay (ELISA) strategy. Prior to assaying, all samples must be treated with an Enhancing Reagent. After treatment with Enhancing Reagent, 8-Isoprostane (15-isoprostane F2t) in the samples or standards is allowed to compete with 8-Isoprostane (15-isoprostane F2t) conjugated to horseradish peroxidase (HRP) for binding to a polyclonal antibody specific for 8-Isoprostane (15-isoprostane F2t) coated on a microplate. Later TMB substrate addition results in a blue color development that is inversely proportional to the quantity of 8-Isoprostane (15-isoprostane F2t) in the original samples or standards. Addition of an acid stop solution causes a color change to yellow where absorbance is read at 450 nM.

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HE Bays, JL Evans, KC Maki, M Evans, V Maquet, R Cooper and JW Anderson
European Journal of Clinical Nutrition ,  2013 Jan;67(1):2-7

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A randomized, double-blind, placebo-controlled, pilot study to evaluate the effect of whole grape extract on antioxidant status and lipid profile
Malkanthi Evans, Dale Wilson, Najla Guthrie
Journal of Functional Foods March 2014; Volume 7, Pages 680–691

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The relationship between work ability and oxidative stress in Japanese workers
Masanori Ohtaa, Masaharu Kumashiro, Yasumasa Eguchi, Yusaku Morita, Yoshimasa Konno & Hiroshi Yamato
Ergonomics 2014; Volume 57, Issue 8, Pages 1265-1273

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Association of altered hemorheology with oxidative stress and inflammation in metabolic syndrome
Prajwal Gyawali; Ross S. Richards
Redox Report May 2015; Volume 20, Issue 3, pp. 139-144

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Association of abnormal erythrocyte morphology with oxidative stress and inflammation in metabolic syndrome
P Gyawali, RS Richards, PT Bwititi, EU Nwose
Blood Cells, Molecules, and Diseases April 2015; Volume 54, Issue 4, Pages 360–363

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For Research Use Only

Catalog Number: NWK-ISO02
Format: 96 Well Competitive ELISA
Sample Requirements: Urine from any species
Specificity: (100.0%) 8-Isoprostane (15-isoprostane F2t)
(4.1%) 9α,11β-Prostaglandin F2α
(3.0%) 13,14-Dihydro-15-Keto-PGF2α
(<0.01%) 9α,11β-Prostaglandin F2α
(<0.01%) Prostaglandin F2α
(<0.01%) 6-Keto-Prostaglandin F1α
(<0.01%) Prostaglandin E2
(<0.01%) Prostaglandin D2
(<0.01%) Arachidonic Acid
Sensitivity: 0.05 ng/mL
Effective Range: .05 ng/mL - 10 ng/mL
Storage and Stability: 6 months from the date of manufacture
Kit Contents: 96-well microtiter plate, precoated with Anti 8-Isoprostane (15-isoprostane F2t)
8-Isoprostane (15-isoprostane F2t) Standard
Enhancer Reagent
5X Wash Buffer
5X Dilution Buffer
TMB Substrate
8-Isoprostane (15-isoprostane F2t) HRP Conjugate
Reagent Trough for multi-channel pipettor
Random Citation | NWK-ISO02

The Effects of Green Tea Extract Supplementation On Delayed Onset Muscle Soreness and Oxidative Stress 
Jordan, SL 
Thesis: M.S.; Texas Tech University August 2007

Link to Abstract

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