Modified Method
- Prepare samples as appropriate
- Dilute the GSHeq calibrator 1/400 and 1/800 (1 µM and 0.5 µM) in Assay Buffer
- Add 50 µL calibrator or sample to microplate well
- Add 50 µL DTNB
- Add 50 µL GR
- Incubate for 2-3 minutes
- Add 50 µL NADPH
- Monitor the 405 nm absorbance
- • Interval = 2-3 minutes
- • Duration = 30 miuntes
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