The NWLSS™ Histamine ELISA kit is intended for use in quantifying the concentration of Histamine in biological samples from multiple species and sample types.
This assay is compatible with samples from all species.
Introduction
Histamine is a heterocyclic primary amine derived from the decarboxylation of the amino acid histidine. It is a mediator of inflammation closely associated with the initial phase of immediate hypersensitivity response (anaphylaxis). Histamine is synthesized by the enzyme histidine decarboxylase and is present in most cells, but typically stored in metachromatic granules of basophils and mast cells (granulocytes) (1). Histamine in the intracellular granules is bound to proteins and inactive until it is released from the cells. Tissue bound mast cells in the skin and nasal mucosa can respond to incoming allergens by releasing histamine which manifests as erythema (redness of skin) and rhinitis (irritation and inflammation) response respectively.
Test Principle
The NWLSS™ Histamine ELISA kit is intended for use in quantifying the concentration of Histamine in biological samples from multiple species and sample types.
For Research Use Only
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Catalog Number:
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NWK-HIS01
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Format:
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96 well competitive ELISA
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Sample Requirements:
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Plasma, serum, tissue homogenates, cell lysates and cell culture supernatatnt from any species
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Specificity:
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Histamine
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Sensitivity:
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2.5 ng/mL
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Intended Use:
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Quantitative measurement of histamine in biological samples.
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Storage and Stability:
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1 year from manufacture date when stored at specified temperature
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Kit Contents:
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Microwells precoated with Anti Histamine
Histamine Standard
25X Concentrated Wash Buffer
Histamine-HRP Conjugate
TMB Substrate
Dilution Buffer
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