Protein Carbonyl ELISA Kit

NWLSS | Northwest Life Science Specialties, LLC.
Protein Carbonyl ELISA Kit
NWK-PCK01 $450.00 each

For questions & ordering, call:
888-449-3091
(Toll-Free US/Canada)



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Protein carbonyls are formed by a variety of oxidative mechanisms and can be used as an index of oxidative injury. The protein carbonyl ELISA Assay kit is recommended as a means to measure generalized oxidation of proteins in plasma, serum, cell lysates, tissue homogenates, BAL, CNS and other biological sample types.

Advantages of the NWLSS™ Protein Carbonyl ELISA Kit:

  • Minimal background signal due to non-specific DNP binding
  • Increased sensitivity for actual sample protein carbonyls
  • Decreased production of artifact protein carbonyls
  • Extremely small sample protein requirements
  • Positive controls are included with each kit
  • Versatility in requisite protein levels
  • Easier to perform protocols
  • Higher throughput

Introduction

The historical method for protein carbonyl analysis was a colorimetric procedure based on the extinction coefficient of a hydrazone compound produced by the reaction of 2,4-dinitrophenylhydrazine (DNPH) with protein carbonyls. In contrast, the Biocell PC Assay utilizes an antibody to directly detect protein carbonyl levels after derivatization and quantify them through comparison to a set of protein carbonyl standards. This method, first published by Hendrikje Buss, Christine Winterbourn et al in 1997 and later commercialized by Zenith Technology Corporation (Zentech) has become the most widely cited assay for protein carbonyl measurement. In recent years, Biocell Corporation has continued to refine the method and carefully optimize it to provide the following benefits compared to other commercially available assays of this type.

For Research Use Only

Catalog Number: NWK-PCK01
Format: 96 Well ELISA
Sample Requirements: Biological fluids such as plasma, serum, bronchoalveolar lavage fluid and cerebrospinal fluid, to cell extracts and to other soluble protein samples.
Specificity: Protein carbonyls
Sensitivity: 0.05 ng/mL
Storage and Stability: Six months from the date of manufacture when stored as specified
Kit Contents: Instructions
ELISA 96-well-plate + sealing tape
EIA buffer powder
Blocking reagent
Dinitrophenylhydrazine
Guanidine hydrochloride diluent
Anti-DNP-biotin-antibody
Streptavidin-horseradish-peroxidase
Chromatin reagent
Stopping reagent
Standards (5)
Carbonyl control samples (low, medium and high)

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