96 well sandwich ELISA for the detection of Oxidized LDL in biological samples. Optimized for human samples however some crossreactivity in rat and pig species has been noted.
Oxidized LDL has been implicated as a potent atherogenic protein primarily found in atherosclerotic lesions of the coronary artery wall. Therefore, it is not only the level of LDL but the "fate" of LDL that adds to increased risk of cardiovascular disease. Oxidized LDL is directly involved in the initiation and progression of the atherosclerotic disease process from the early-stage conversion of monocytes/macrophages into lipid-laden foam cells, to the late-stage development of coronary artery stenosis, plaque instability, plaque rupture, coronary thrombosis, and myocardial infarction.
With this in mind it is easier to understand why it is that not all populations with high cholesterol diets show an equally high rate of cardiovascular disease. The redox status of the circulatory system and the level of inflammation and overall immune response at affected sites in the coronary artery wall are likely equally important (possibly more) than the actual level of LDL itself.
The Ox-LDL ELISA kits we offer are manufactured by Mercodia AB, and can now be purchased direct from Northwest Life Science Specialties, LLC. Both the Sandwich and Competive format OxLDL ELISA kits utilize the same antibody used by Hoelvet, et al 1998 and 2001. The Sandwich ELISA also utilizes an anti-apo B detection antibody.
View Assay Protocol
96 Well Sandwich ELISA
of oxidized low density lipoproteins (oxidized LDL) in human blood serum or plasma.
EDTA and Heparinized Plasma or Serum
Oxidized Low Density Lipoprotein (LDL)
1mU/L (three standard deviations from zero calibrator)
|Storage and Stability:||
Anti-Oxidized LDL Coated Microtitre Plate, (12 X 8 well strips)|
Oxidized LDL Standards, 5 vials lyophilized
Oxidized LDL Zero Standard, 1 vial, 1.0 mL
Oxidized LDL Controls, 2 Vials, Low & High
11X Anti-apo-B/Enzyme Conjugate, 1 Vial, 1.2 mL
Conjugate Buffer, 1 Vial, 12 mL
Assay Buffer 1 vial, 12 mL
Sample Buffer, 2 Bottles, 100 mL
21X Wash Buffer, 1 Bottle, 40 mL
TMB Substrate, 1 Vial, 22 mL
Stop solution 1 vial, 7 mL