Nrf2 (NFE2L2) ELISA Kits

NWLSS | Northwest Life Science Specialties, LLC.
NWK-NRF2H1 $845.00 each

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The NWLSS™ Nrf2 (NFE2L2) ELISA is intended for quantitative detection of Nuclear Factor Erythroid 2 Like Protein 2, commonly known as Nrf2. The assay comes in three variants, specific for either the human, mouse, or rat isoforms. Each variant does not crossreact with the others.
 
Variants of the NWLSS™ Nrf2 ELISA:

  • Human [NWK-NRF2H1]
  • Mouse [NWK-NRF2M1]
  • Rat [NWK-NRF2R1]

Introduction

Nrf2 is a basic leucine zipper (bZIP) protein functioning to regulate the expression of many antioxidant proteins. Nrf2 can be found in the cytosol where it is bound to the Kelch-like ECH-associated protein 1 (KEAP1)regulatory molecule. In this sequestered condition Nrf2 cannot cross into the nucleus. However, under conditions of oxidative stress, KEAP1 can dissociate from Nrf2 allowing NRf2 to translocate into the nucleus. Inside the nucleus Nrf2 binds to small Maf (sMAF) proteins forming an Nrf2/sMaf heterodimer capable of binding to the Antioxidant Response Element (ARE) on various stress related gene targets.

In recent years, Nrf2/KEAP1 regulation of the Nrf2/sMAF/ARE transcription pathway has been investigated extensively to assess its possible role in aging and disease. Many studies are showing a positive correlation between Nrf2 nuclear translocation and amelioration of disease resulting in increased interest in Nrf2 promotors and KEAP1 agonists as possible therapeutic agents. Other studies show paradoxical outcomes such as when considering Nrf2 in relation to cancer. In that case, it appears that some tumor cells are capable of exploiting the Nrf2 system to upregulate antioxidant expression which seems to confer chemo-protective properties making the tumor more virulent and resistant to therapy.

In either role Nrf2 has emerged as a very important target for researchers attempting to better understand the positive and negative effects of redox related stress response.

Test Principle

The NWLSS™ Nrf2 (NFE2L2) ELISA is a sandwich format Enzyme-Linked Immunosorbent Assay (ELISA). The assay features a microtiter plate pre-coated with a monoclonal antibody specific to either human, mouse, or rat Nrf2.

Briefly, Nrf2 in samples and standards is captured by the plate bound antibody and remaining liquid is removed. Next, a biotinylated antibody with Nrf2 specificity is added and allowed to bind to Nrf2 captured in the previous step. After incubation, non-bound components are removed by washing. Avidin-HRP conjugate is then added and allowed to incubate. After a subsequent thorough wash TMB-substrate solution is added to each well which produces blue coloration wherever Nrf2 is present. Finally, a sulfuric acid stop solution is added and the resulting yellow colored product is measured at 450nm.

The amount of Nrf2 in the sample can be easily determined by direct comparison of unknown sample absorbance with known standard curve absorbance values generated in the assay.

For Research Use Only

Catalog Number: NWK-NRF2H1
Format: 96 Well ELISA
Sample Requirements: Tissue Homogenates or Cell Lysates
Specificity: Human NRF2 (NFE2L2)
Sensitivity: 55 pg/mL
Standard Range: 0.156 ng/mL - 10 ng/mL
Storage and Stability: 6 months from the date of manufacture when individual components stored as specified.
Kit Contents: 1 12x8 Well Foil Pouch Microtiter plate precoated with anti-Human Nrf2
2 Vials NFE2L2 (Nrf2) Standard (Lyophilized)
1 Bottle Standard Diluent (20 mL)
1 Vial 100X Secondary Antibody (120 µL)
1 Bottle Secondary Antibody Diluent (12 mL)
1 Vial 100X Avidin-HRP Conjugate (120 µL)
1 Bottle Avidin-HRP Diluent (12 mL)
1 Bottle TMB Substrate (9 mL)
1 Bottle Stop Solution (6 mL)
1 Bottle 30X Wash Buffer (20 mL)
4 Adhesive Plate Covers
Catalog Number: NWK-NRF2M1
Format: 96 Well ELISA
Sample Requirements: Tissue Homogenates or Cell Lysates
Specificity: Mouse NRF2 (NFE2L2)
Sensitivity: 6.0 pg/mL
Standard Range: 15.6 pg/mL - 1 ng/mL
Storage and Stability: 6 months from the date of manufacture when individual components stored as specified.
Kit Contents: 1 12x8 Well Foil Pouch Microtiter plate precoated with anti-Mouse Nrf2
2 Vials NFE2L2 (Nrf2) Standard (Lyophilized)
1 Bottle Standard Diluent (20 mL)
1 Vial 100X Secondary Antibody (120 µL)
1 Bottle Secondary Antibody Diluent (12 mL)
1 Vial 100X Avidin-HRP Conjugate (120 µL)
1 Bottle Avidin-HRP Diluent (12 mL)
1 Bottle TMB Substrate (9 mL)
1 Bottle Stop Solution (6 mL)
1 Bottle 30X Wash Buffer (20 mL)
4 Adhesive Plate Covers
Catalog Number: NWK-NRF2R1
Format: 96 Well ELISA
Sample Requirements: Tissue Homogenates or Cell Lysates
Specificity: Rat NRF2 (NFE2L2)
Sensitivity: 60 pg/mL
Standard Range: 0.156 ng/mL - 10 ng/mL
Storage and Stability: 6 months from the date of manufacture when individual components stored as specified.
Kit Contents: 1 12x8 Well Foil Pouch Microtiter plate precoated with anti-Rat Nrf2
2 Vials NFE2L2 (Nrf2) Standard (Lyophilized)
1 Bottle Standard Diluent (20 mL)
1 Vial 100X Secondary Antibody (120 µL)
1 Bottle Secondary Antibody Diluent (12 mL)
1 Vial 100X Avidin-HRP Conjugate (120 µL)
1 Bottle Avidin-HRP Diluent (12 mL)
1 Bottle TMB Substrate (9 mL)
1 Bottle Stop Solution (6 mL)
1 Bottle 30X Wash Buffer (20 mL)
4 Adhesive Plate Covers

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