How should tissues be homogenized for assay using the NWK-NTR02-01 kit? We normally recommend that tissue samples re homogenized in normal PBS, pH 7.0. Homogenization can be performed with a dounce style or Potter Elvehjem style tissue grinder or similar apparatus. Tissue homogenates should be centrifuged and the clarified supernatant tested for nitrotyrosine. Several references wherein other researchers have used the NWK-NTR01 kit to test tissue samples can be found in the citations references for this product.