Human Thioredoxin Reductase 1 (TrxR1) ELISA Kit

NWLSS | Northwest Life Science Specialties, LLC.
nwk-trr01
NWK-TRR01 $595.00 each

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NWK-TRR01
The NWLSS™ TrxR1 ELISA is intended for the quantitative detection of human Thioredoxin Reductase 1 (TrxR1) in biological samples where it may be present.

Introduction

The mammalian thioredoxin reductases (TrxRs) are a family of selenocysteine containing pyridine nucleotide-disulfide oxido-reductases. Three different TrxR isoenzymes, TrxR1 as cytosolic, TrxR2 as mitochondrial, and TrxR3 as testis-specific thiol regulator are known. All the mammalian TrxRs are homologous to glutathione reductase with respect to primary structure including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) but distinctively with a C-terminal extension containing a catalytically active penultimate selenocysteine(SeCys) residue in the conserved sequence(-Gly-Cys-SeCys-Gly). TrxR is homodimeric protein in which each monomer includes an FAD prosthetic group, a NADPH binding site and a redox catalytic site. Electrons are transferred from NADPH via FAD and the active-site disulfide to C-terminal SeCys-containing redox center, which then reduces the substrate like thioredoxin. The members of TrxR family are 55�58 kDa in molecular size and composed of three isoforms including cytosolic TrxR1, mitochondrial TrxR2, and TrxR3, also known as Trx and GSSG reductase (TGR). TrxR plays a key role in protection of cells against oxidative stress and redox-regulatory mechanism of transcription factors and various biological phenomena. Many tumor cells have elevated TrxR levels and TrxR has been shown to play a major role in drug resistance. Inhibition of TrxR and its related redox reactions may thus contribute to a successful single, combinatory or adjuvant cancer therapy. A great number of effective natural and synthetic TrxR inhibitors are now available possessing antitumor potential ranging from induction of oxidative stress to cell cycle arrest and apoptosis.

Test Principle

The NWLSS™ Thioredoxin Reductase 1 (TrxR1) ELISA kit is to be used for the in vitro quantitative determination of human TrxR1 in human plasma, cell lysate and buffered solution. The assay will recognize both native and recombinant human TrxR1.

View Assay Protocol

Catalog Number: NWK-TRR01
Format: 96 wells presented as 1 plate plus frame sectionable as 16 well individual strips
Intended Use Quantitative measurement of human TrxR1 in biological samples.
Sample Requirements: Human plasma and other biological fluids where Thioredoxin Reductase 1 may be present
Specificity: Human Thioredoxin Reductase 1
Sensitivity: 0.4 ng/mL
Storage and Stability: 1 year from manufacture date when stored at specified temperature
Kit Contents: Microwell strips precoated w/anti huTrxR1 and strip frame
rHu-Thioredoxin Reductase 1 Standard (lyophilized)
Sample Dilution Buffer
100X Secondary Antibody (biotin labeled anti-huTrxR1)
Reagent Dilution Buffer
100X Avidin-HRP Conjugate
Assay Preparation Buffer
TMB Substrate
Stop Solution
10X Concentrated Wash Buffer
Adhesive Plate Covers

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