Human Thioredoxin-1 (Trx1) ELISA Kit

NWLSS | Northwest Life Science Specialties, LLC.
nwk-trx01
NWK-TRX01 $595.00 each

For questions & ordering, call:
888-449-3091
(Toll-Free US/Canada)



+
NWK-TRX01
Trx is a multifunctional protein with anti-inflammatory and anti-apoptotic effects, as well as antioxidative effects. Accordingly, Trx is a well-recognized biomarker of various diseases involving oxidative stress and is a useful biomarker for research focused in this area

Introduction

Thioredoxins(Trx) are small, multi-functional proteins with oxido-reductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Thioredoxin reductase (TR) with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide�isomerase (PDI) and a number of transcription factors such as p53, NF-kB and AP-1 (T1-151). Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins. To summarize, Trx is a multifunctional protein with anti-inflammatory and antiapoptotic effects, as well as antioxidative effects. Accordingly, Trx is a well-recognized biomarker of various diseases involving oxidative stress and is a useful biomarker for research focused in this area.

Test Principle

The NWLSS™ Thioredoxin 1 (human Trx1) ELISA kit is to be used for the in vitro quantitative determination of human Trx1 in cell lysate and buffered solution. The assay will recognize both native and recombinant human Trx1.

View Assay Protocol

Catalog Number: NWK-TRX01
Format: 96 wells presented as 1 plate sectionable as 8 well individual strips
Intended Use Quantitative measurement of human Trx1 in biological samples.
Sample Requirements: Human plasma and other biological fluids where Thioredoxin 1 may be present
Specificity: Human Thioredoxin-I
Sensitivity: 0.4 ng/mL to 25 ng/mL
Storage and Stability: 1 year from manufacture date when stored at specified temperature
Kit Contents: Microwell strips precoated w/anti huTrx1 and strip frame
rHu-Thioredoxin 1 Standard (lyophilized)
Sample Dilution Buffer
100X Secondary Antibody (biotin labeled anti-huTrx1)
Reagent Dilution Buffer
100X Avidin-HRP Conjugate
Assay Preparation Buffer
TMB Substrate
Stop Solution
20X Concentrated Wash Buffer
Adhesive Plate Covers

For questions, orders, and technical service:

 
360-449-3091
(Direct)
 
888-449-3091
(Toll-Free US/Canada)
 
360-397-0181
(Fax)
Online Support