Human Peroxiredoxin 3 (Prx3) ELISA Kit

NWLSS | Northwest Life Science Specialties, LLC.
nwk-prx03
NWK-PRX03 $595.00 each

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NWK-PRX03
The NWLSS™ Peroxiredoxin 3 (Prx3) ELISA kit is to be used for the in vitro quantitative determination of human Prx3 in serum, plasma, cell lysate and other biological samples. The assay will recognize both native and recombinant human Prx3.

Introduction

Mammals have 6 isoforms of the anti oxidative protein Peroxiredoxin (Prx). The specific localization of Prx3 in mitochondria together with the electron donors, thioredoxin 2 and thioredoxin reductase 2, suggest that these three proteins may provide a primary line of defense against H2O2 produced by the mitochondrial respiratory chain. Organisms living under aerobic conditions have developed various anti-oxidative mechanisms to protect them from damage by reactive oxygen species (ROS). Peroxiredoxin (Prx) is a growing anti-oxidative protein family that has been identified six members in mammals. They share a common reactive Cys residue in the N-terminal region, and are capable of serving as a peroxidase and involve thioredoxin and/or glutathione as the electron donor. Prx1 to Prx4 have an additional Cys residue in the conserved C-terminal region, and are cross members as judged by the amino acid sequence similarity. Prx5 also contains an additional Cys in its C-terminal region that is less conserved. On the other hand, Prx6 has only one unique Cys. These Prx family members are distributed in subcellular localization, Prx1, 2, and 6 in cytosol, Prx3 in mitochondria, Prx4 in ER and secretion, Prx5 showing complicated distribution including peroxisome, mitochondria and cytosol, all of which are potential sites of ROS production. In addition to their role as a peroxidase, however, a body of evidence has accumulated to suggest that individual members also serve divergent functions, which are associated with various biological processes such as the detoxification of oxidants, cell proliferation, differentiation and gene expression. It would be expected that these functions might not necessarily depend on peroxidase activity and, therefore, it seems likely that the divergence is due to unique molecular characteristics intrinsic to each member. The specific localization of Prx3 in mitochondria together with the identification of its mitochondria-specific electron suppliers, namely thioredoxin 2 and thioredoxin reductase 2, suggest that these three proteins might provide a primary line of defense against H2O2 produced by the mitochondrial respiratory chain. Furthermore, Prx3 expression is induced by oxidants in the cardiovascular system and is thought to play a role in the antioxidant defense system and homeostasis within mitochondria.

Test Principle

The NWLSS™ Peroxiredoxin 3 (Prx3) ELISA kit is to be used for the in vitro quantitative determination of human Prx3 in serum, plasma, cell lysate and other biological samples. The assay will recognize both native and recombinant human Prx3.

View Assay Protocol

Catalog Number: NWK-PRX03
Format: 96 wells presented as 1 plate sectionable as 8 well individual strips
Intended Use Quantitative measurement of human Prx3 in biological samples.
Sample Requirements: Human plasma and other biological fluids where Peroxiredoxin 3 may be present
Specificity: Human Peroxiredoxin 3
Sensitivity: 2 ng/mL to 32 ng/mL
Storage and Stability: 1 year from manufacture date when stored at specified temperature
Kit Contents: Microwell strips precoated w/anti huPrx3 and strip frame
rHu-Peroxiredoxin 3 Standard (lyophilized)
Sample Dilution Buffer
100X Secondary Antibody (biotin labeled anti-huPrx1)
Reagent Dilution Buffer
100X Avidin-HRP Conjugate
Assay Preparation Buffer
TMB Substrate
Stop Solution
10X Concentrated Wash Buffer
Adhesive Plate Covers

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