Human Myeloperoxidase (MPO) ELISA Kit: 192

NWLSS | Northwest Life Science Specialties, LLC.
nwk-mpo02
NWK-MPO02 $1,045.00 each

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NWK-MPO02
MPO has been implicated as playing a role in the oxidation of low density lipoprotein (LDL) and the advancement of cardiovascular disease. This kit has been optimized for measurement of MPO "expression" in human plasma and tissue samples. Cross reactivity in rodent model systems has not been tested. The activity of MPO enzyme from any species can be tested using product number NWK-MPO03.

Introduction

Myeloperoxidase (MPO) is an iron containing protein tetramer expressed in all cells of the myeloid linage. MPO is abundantly present in azurophilic granules of polymorphonuclear neutrophils. It is an important enzyme used during phagocytic lysis of engulfed foreign particles which takes part in the defense of the organism through production of hypochlorous acid (HOCl), a potent oxidant. MPO is rapidly released by activated neutrophils. Involvement of MPO has been described in numerous diseases such as atherosclerosis, lung cancer, Alzheimer's disease and multiple sclerosis. Autoimmune antibodies to MPO are involved in Wegener's disease. Since the discovery of MPO deficiency, initially regarded as rare and restricted to patients suffering from severe infections, MPO has attracted more clinical attention. Today it is regarded as one of the leading candidates for prognosis of cardiac disease. The classical MPO assay is an enzymatic assay for activity which can be hampered by the presence of inhibitory compounds in many tissue homogenates. The NWLSS™ MPO assay utilizes a unique buffer system to reduce the possible effects of loss of enzyme activity on enzyme detection.

View Assay Protocol

Catalog Number: NWK-MPO02
Format: Enzyme Immunoassay
Intended Use Measurement of human MPO in plasma, cell culture medium and other biological fluids where MPO may be present
Sample Requirements: Plasma, tissue & homogenates, and other biological fluids where MPO may be present
Specificity: Human Myeloperoxidase
Sensitivity: 0.4 ng/mL
Storage and Stability: Twelve months from date of manufacture when stored at 2-8C
Kit Contents: Precoted Microwell strips and strip frames
Wash Buffer
Dilution Buffers A & B
Myeloperoxidase Standard
Biotinylated anti myeloperoxidase tracer
Streptavidin Peroxidase
TMB Substrate
Stop Solution

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