The NWLSS™ Human IL-10 ELISA kit is intended to be used for the in vitro quantitative determination of Interleukin 10 (IL-10) in human serum, plasma, cell lysates and cell culture supernatants. The assay will recognize native and recombinant human IL-10.
Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immune-regulatory cytokine that plays an important role in the development of infectious disease. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.
The NWLSS™ Human IL-10 ELISA is a sandwich format Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to human IL-10. Samples are pipetted into these wells. Non-bound IL-10 and other components of the sample are removed by washing, after which a biotinylated antibody specific to human IL-10 is added. In order to quantitatively determine the amount of IL-10present in the sample, Streptavidin Horseradish Peroxidase (HRP) conjugate is added to each microplate well. After another wash step, TMB-substrate solution is added to each well. Finally, a sulfuric acid stop solution is added and the resulting yellow colored product is measured at 450nm. The amount of IL-10 in the sample can be determined by direct comparison with the
standard curve generated in the assay.
View Assay Protocol
96 well ELISA presented as 12 X 8 well (6 X 16 well) strips in frame.
For quantitative detection of human IL-10.
Human serum, plasma, cell lysates and cell culture supernatants.
|Storage and Stability:||
9 months from date of manufacture
1 Foil Pouch 96 well microplate precoated with anti-Human IL-10|
2 bottles 20X Concentrated Wash Buffer (25 mL)
1 vial rHu IL-10 Standard (lyophilized) (1 Vial)
1 bottle Standard/Sample Dilution Buffer (25mL)
1 vial Secondary Antibody (Lyophilized) (1 Vial)
(Biotinylated Anti-Hu IL-10)
1 vial 100X Streptavidin-HRP Conjugate (150 µL)
1 bottle Reagent Dilution Buffer (25mL)
1 bottle TMB Substrate (15 mL)
1 bottle Stop Solution (1 N Sulfuric Acid, H2SO4) (15 mL)
2 Adhesive Plate Covers (2)