Human Extracellular Superoxide Dismutase (ecSOD or SOD3) ELISA Kit

NWLSS | Northwest Life Science Specialties, LLC.
nwk-sod03
NWK-SOD03 $595.00 each

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NWK-SOD03
The NWLSS™ ecSOD ELISA is intended for the quantitative detection of human Extracellular Superoxide Dismutase (SOD3) in biological samples where ecSOD may be present.

Introduction

Superoxide dismutase (SOD) is an antioxidative enzyme involved in the defense against reactive oxygen species (ROS). SOD catalyzes the dismutation of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and/or catalase. Three unique and highly compartmentalized mammalian superoxide dismutases have been biochemically and molecularly characterized. SOD1, or CuZnSOD or SOD1 exists as a homodimer with Cu/Zn at its active site. Cu/ZnSOD is found almost exclusively in the intracellular cytosol. MnSOD or SOD2 exists as a tetramer with Mn at the active site. MnSOD is initially synthesized with a leader peptide which targets this enzyme exclusively to the mitochondrial spaces. EcSOD or SOD3 is the most recently characterized SOD. Although EcSOD also has Cu/Zn at its active site, it exists as a tetramer and contains a unique heparin-binding domain at its carboxy-terminus that enables its localization to the extracellular matrix. EcSOD is highly expressed in selected tissues including blood vessels, heart, lungs, kidney and placenta where it is found in the interstitial extracellular matrix. EcSOD is now known to play an important role in maintaining vascular tone, attenuating age-related cognitive decline, lung function, and the metabolism of NO.

Test Principle

The NWLSS™ ecSOD Assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to human ecSOD. This stationary phase antibody binds sample or standard ecSOD while nonbound proteins are removed by washing. Next, bound ecSOD is tagged with a biotin-conjugated monoclonal antibody specific for ecSOD followed by Avidin conjugated to Horseradish Peroxidase (HRP). Subsequent addition of TMB-substrate solution causes blue color (650 nm) development proportional to the amount of ecSOD originally captured by the stationary phase antibody. Finally, addition of a sulfuric acid solution stops the reaction resulting in a yellow color product measured at 450 nm. Sample ecSOD concentration is determined by comparing the 450 nm absorbance of sample wells to the absorbance of known standards.

View Assay Protocol

Catalog Number: NWK-SOD03
Format: 96 Well ELISA (Sandwich)
Intended Use Quantitative determination of human extracellular SOD in cell lysate and tissue homogenates
Sample Requirements: Human cell lysate or tissue homogenate
Specificity: Human EcSOD
Sensitivity: 2 ng/mL
Storage and Stability: 1 year from manufacture date when stored at specified temperature
Kit Contents: Precoated Microwell Strips
rHu ecSOD Standard
Sample/Standard Dilution Buffer
Reagent Dilution Buffer
Assay Preparation Buffer
Avidin-HRP Conjugate
Biotin Labeled anti hu ecSOD
TMB Substrate
Stop Solution

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