Human Cytosolic Superoxide Dismutase (Cu/ZnSOD, SOD1) ELISA Kit

NWLSS | Northwest Life Science Specialties, LLC.
nwk-sod01
NWK-SOD01 $595.00 each

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NWK-SOD01
The NWLSS™ Cu/ZnSOD ELISA is intended for the quantitative detection of human Cu/ZnSOD in human plasma, serum cell culture supernatants, erythrocyte (RBC) or other cell lysates and other biological fluids where SOD may be present.

Introduction

Superoxide Dismutases (SODs) are a unique family of metalloproteins that catalyze the dismutation of superoxide anion radicals (O2-) to oxygen (O2) and hydrogen peroxide (H2O2). SOD protects cells against oxygen-mediated free radical damage. Four types of SOD have been defined on the basis of their metal cofactors and distribution. The Cu/Zn form is the most common, with a primary distribution in the cytoplasm of of eukaryotic cells. The manganese form (MnSOD) is generally associated with the mitochondria of aerobic organisms. The iron (FeSOD) form is found predominantly in prokaryotes. The most recently discovered form, extra-cellular (ecSOD) is found in the extra-cellular fluids of mammalian species, often associated with vascular tissues.

Test Principle

The NWLSS™ Cu/ZnSOD ELISA is intended for the quantitative detection of human Cu/ZnSOD in human plasma, serum cell culture supernatants, erythrocyte (RBC) or other cell lysates and other biological fluids where SOD may be present.

View Assay Protocol

Catalog Number: NWK-SOD01
Format: 96 wells presented as 1 plate sectionable as 8 well individual strips
Intended Use Quantitative measurement of human Cu/ZnSOD in biological samples.
Sample Requirements: Human plasma, serum cell culture supernatants, erythrocyte (RBC) or other cell lysates
Specificity: Human Cu/Zn Superoxide Dismutase
Sensitivity: 0.04 ng/mL (40 pg/mL)
Storage and Stability: 1 year from manufacture date when stored at specified temperature
Kit Contents: Precoted Microwell strips and strip frame
Concentrated Wash Buffer
Concentrated Assay Buffer
Cu/ZnSOD Standard
Concentrated Phosphate Buffered Saline
Anti-Cu/ZnSOD-HRP Conjugate
TMB Substrate Solution I
Substrate Solution II
Stop Solution

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