Glutathione Reductase (GR) Assay Kit: 192 Tests

NWLSS | Northwest Life Science Specialties, LLC.
Glutathione Reductase (GR) Assay Kit
NWK-GR01 $395.00 each

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The NWLSS™ Glutathione Reductase Assay kit is a kinetic, rate based assay for quantification of glutathione reductase (GR) in a variety of samples including blood cells, tissues and cultured cells.

Introduction

Glutathione Reductase is a ubiquitous 100-120 kD dimeric flavoprotein that catalyzes the reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH) using b-nicotinamide dinucleotide phosphate (NADPH) as the hydrogen donor. A measurable fraction of GR derived from various sources is often found as the inactive apoenzyme. Dietary supplementation of riboflavin or thyroxin has been shown to activate GR. The in vitro addition of FAD is the basis of assessing riboflavin deficiency. The active site for GR contains a flavin adenine dinucleotide (FAD) and a disulfide. In the presence of NADPH, there is a two electron reduction of GR to produce a semiquinone of FAD, a sulfur radical and a thiol. Purified GR tends to form aggregates in the absence of thiols and these aggregates retain full enzymatic activity. Purified enzyme is reversibly inactivated by NADPH, m-nicotinamide adenine dinucleotide (NADH), GSH, dithionite or borohydride. This inactivation likely requires the presence of divalent cations such as Zn++ and Cd++. The GR enzyme is fully protected from inactivation by ethylenediamine tetraacetic acid (EDTA). Inactivated GR is activated by GSSG, NADP+ and NAD+. In vivo, GR activity is regulated through a redox interconversion mechanism mediated by GSSG regulation of the NADPH generating pathways.

Test Principle

The most widely used procedure to measure GR is to monitor the oxidation of NADPH as a decrease in absorbance at 340 nm as shown in Figure 1.

Reduction of GSSG by NADPH catalyzed by GR

For each mole of GSSG reduced, one mole of NADPH is oxidized resulting in loss of absorbance at 340 nm. One Unit GR activity is defined as the amount of enzyme that will reduce 1 mmole GSSG per minute at pH 7.6 and 25C.

Can I use the same deproteinated sample I am testing for Glutathione (GSH) to test for Glutathione Reductase?

No. Glutathione reductase is a protein and will be removed by deproteination. It would be necessary to split the sample prior to deproteination then test GPx in the non-deproteinated sample.

Effect of Silver Nanoparticles on the Brain of Oreochromis niloticus and Tilapia zillii
Afifi M, Saddick S, Zinada OAA 
Saudi Journal of Biological Sciences Available online July 4, 2016

Link to Abstract

Dietary zinc deficiency induces oxidative stress and promotes tumor necrosis factor-α- and interleukin-1β-induced RANKL expression in rat bone
Takako Suzuki1), Shin-ichi Katsumata1), Hiroshi Matsuzaki1), Kazuharu Suzuki1)
Journal of Clinical Biochemistry and Nutrition,  (February 2016) : Available Online

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Ameliorative effect of zinc oxide nanoparticles on antioxidants and sperm characteristics in streptozotocin-induced diabetic rat testes
Mohamed Afifi, Omar A. Almaghrabi and Naif Mohammed Kadasa
Biomed Research International October 11 2015; Published Online

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Quercetin and Omega 3 Ameliorate Oxidative Stress Induced by Aluminium Chloride in the Brain
Haytham Abdallah Ali, Mohamed Afifi, Aaser Mohamed Abdelazim, Yahia Youssef Mosleh
Journal of Molecular Neuroscience February 2014; Published Online Ahead of Print

Link to Abstract

Protective Effects of Tualang Honey against Oxidative Stress and Anxiety-Like Behaviour in Stressed Ovariectomized Rats
Badriya Al-Rahbi, Rahimah Zakaria, Zahiruddin Othman, Asma’ Hassan, and Asma Hayati Ahmad
International Scholarly Research Notices September, 2014; Volume 2014, Article ID 521065, 10 pages

Link to Abstract

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For Research Use Only

Catalog Number: NWK-GR01
Format: 192 well microplate (2 plates).
Intended Use Quantification of GR enzyme activity in biological samples such as tissue homogenates and cell lysates.
Sample Requirements: Tissue and cell lysates
Specificity: Glutathione Reductase Activity
Sensitivity: LLD = 1.5 mU/mL
Storage and Stability: 9 months from date of manufacture if stored as specified
Kit Contents: NADPH Reagent NADPH Diluent GSSG Reagent GR Calibrator Assay Buffer Microplates
Random Citation | NWK-GR01

Protective Effects of Tualang Honey against Oxidative Stress and Anxiety-Like Behaviour in Stressed Ovariectomized Rats
Badriya Al-Rahbi, Rahimah Zakaria, Zahiruddin Othman, Asma’ Hassan, and Asma Hayati Ahmad
International Scholarly Research Notices September, 2014; Volume 2014, Article ID 521065, 10 pages

Link to Abstract

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