Anti-Thioredoxin 1 (TRx1) Monoclonal

NWLSS | Northwest Life Science Specialties, LLC.
nwa-trx103a1
NWA-TRX103A1 $375.00 each

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Introduction

Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity that are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cystein residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds (1). Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations (2). Specific protein disulfide targets for reduction by Trx include protein disulfide -isomerase (PDI) (3) and a number of transcription factors such as p53 (4), NF-kB (5) and AP-1 (T1-151). Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins (6-7).


Host: Murine
Clonality: Monoclonal
Isotype: IgG2b, k
Immunogen: Recombinant human protein purified from E.coli
Cross Reactivity
Human
Application(s): ELISA
Western Blot: (1:2000)
Immunoprecipitation
IHC
Storage/Stability:
Stable for 1 years at -20 °C
Packaging Size:
100 μL
Format:
Liquid
Concentration:
1 mg/mL

References:
1) Andoh, T. et al. (2002) J. Biol. Chem. 277, 9655-9660.
2) Arner, E. S. and Holmgren, A. (2000) Eur. J. Biochem. 267, 6102-6109.
3) Lundstrom, J. and Holmgren, A. (1990) J. Biol. Chem. 265, 1994-9120. 
4) Nordberg, J. and Arner, E. S. J. (2001) Free Radic. Biol. Med. 31
1287-1312
5) Matthews, J. R. et al. (1992) Nucleic Acids Res. 20, 3821-3830.
6) Wei, S. J. (2000) Cancer Res. 60, 6688-6695.
7) Chae, H. Z. (1999) Methods Enzymol. 300, 
219-226.

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