Summary

Protein carbonyls are formed through oxidation of proteins by a variety of mechanisms. They are sensitive markers of oxidative injury. The customary way of analysing protein carbonyls is a colorimetric procedure that measures binding of dinitrophenylhydrazine (DNP). This test is an ELISA format using an anti-DNP antibody developed by: I. Hendrikje Buss, Christine C. Winterbourn and coworkers at the Free Radical Research Group, Christchurch School of Medicine (University of Otago), Christchurch, New Zealand.

Key Benefits:

• Measures carbonyls quantitatively
• Requires microgram amounts of protein
• Has a very low non-specific background
• Is highly sensitive for analysing clinical samples
• Correlates directly with the classical colorimetric assay
• Is easier to use, less labour-intensive and handles more samples per day than the colorimetic assay
• Strip-well plates allow partial use of the kit for smaller runs