Myeloperoxidase(MPO) is a hemoprotein that is abundantly expressed in polymorphonuclear leukocytes (neutrophils) and secreted during their activation. Native MPO is a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59 ~ 64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW about 150 kDa. MPO plays an important role in neutrophil related anti-microbial action by catalyzing the oxidation of chloride ion to hypochlorous acid. MPO is also now thought to play a role in several autoimmune diseases as well as in cardiovascular disease by contributing to the severity of atherosclerotic inflammatory lesions.
The NWLSS™ Myeloperoxidase (MPO) Assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to human MPO. This stationary phase antibody binds sample or standard MPO while nonbound proteins are removed by washing. Next, bound MPO is tagged with a biotin-conjugated monoclonal antibody specific for MPO followed by Avidin conjugated to Horseradish Peroxidase (HRP). Subsequent addition of TMB-substrate solution causes blue color (650 nm) development proportional to the amount of MPO originally captured by the stationary phase antibody. Finally, addition of a sulfuric acid solution stops the reaction resulting in a yellow color product measured at 450 nm. Sample MPO concentration is determined by comparing the 450 nm absorbance of sample wells to the absorbance of known standards.
||96 Well Sandwich ELISA (6 X 16 well strips in frame)
||Human plasma, cell lysate or tissue homogenate
||Precoated Microwell Strips
rHu MPO Standard
Sample/Standard Dilution Buffer
Reagent Dilution Buffer
Assay Preparation Buffer
Biotin Labeled anti hu MPO