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Human Myeloperoxidase (MPO) ELISA...96 Well Version using Monocl
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Human Myeloperoxidase (MPO) ELISA...96 Well Version using Monocl

Price: $645.00

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NWK-MPO04
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Summary

Myeloperoxidase(MPO) is a hemoprotein that is abundantly expressed in polymorphonuclear leukocytes (neutrophils) and secreted during their activation.  Native MPO is a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59 ~ 64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW about 150 kDa.  MPO plays an important role in neutrophil related anti-microbial action by catalyzing the oxidation of chloride ion to hypochlorous acid.  MPO is also now thought to play a role in several autoimmune diseases as well as in cardiovascular disease by contributing to the severity of atherosclerotic inflammatory lesions.

Method

The NWLSS™ Myeloperoxidase (MPO) Assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA).  The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to human MPO.  This stationary phase antibody binds sample or standard MPO while nonbound proteins are removed by washing.  Next, bound MPO is tagged with a biotin-conjugated monoclonal antibody specific for MPO followed by Avidin conjugated to Horseradish Peroxidase (HRP).  Subsequent addition of TMB-substrate solution causes blue color (650 nm) development proportional to the amount of MPO originally captured by the stationary phase antibody.  Finally, addition of a sulfuric acid solution stops the reaction resulting in a yellow color product measured at 450 nm.  Sample MPO concentration is determined by comparing the 450 nm absorbance of sample wells to the absorbance of known standards.
Catalog Number: NWK-MPO04
Format: 96 Well Sandwich ELISA (6 X 16 well strips in frame)
Sample Requirements: Human plasma, cell lysate or tissue homogenate
Sensitivity: 0.78 ng/mL
Kit Contents: Precoated Microwell Strips
rHu MPO Standard
Sample/Standard Dilution Buffer
Reagent Dilution Buffer
Assay Preparation Buffer
Avidin-HRP Conjugate
Biotin Labeled anti hu MPO
TMB Substrate
Stop Solution