Oxidative DNA Biomarkers

Catalog: NWK-8OHDG01
NWLSS™ Urinary 8-OHDG ELISA
8-hydroxy-2'-deoxyguanosine (8-OHdG) is formed when DNA is oxidatively modified by reactive oxygen species (ROS). 8-OHdG is one of the most sensitive biomarkers for oxidative stress and can be detected in various biological sample types. The NWLSS™ Urinary 8-OHDG ELISA is intended for quantitative detection of the 8-OHdG adduct in urine.  We recommend product number NWK-8OHDG02 for testing samples expected to contain lower amounts of 8-OHdG such as plasma, and/or DNA extracts isolated from cell lysates or tissue homogenates.

Catalog: NWK-8OHDG02
NWLSS™ 8-OHDG ELISA
8-hydroxy-2'-deoxyguanosine (8-OHdG) is formed when DNA is oxidatively modified by reactive oxygen species (ROS). 8-OHdG is one of the most sensitive biomarkers for oxidative stress and can be detected in various biological sample types. The NWLSS™ 8-OHDG ELISA is intended for quantitative detection of the 8-OHdG adduct in plasma, and/or DNA extracts isolated from cell lysates or tissue homogenates. .  When testing urine samples, same day results are possible when using product number NWK-8OHDG01. or tissue homogenates.

Oxidative Lipid Biomarkers

Catalog: NWK-HEXL01
NWLSS™ Hexanoyl-Lysine ELISA
Oxidative damage to lipids (lipid peroxidation) has been found to play an important role in various disease and aging processes. During early stages of lipid peroxidation, lipid hydroperoxides (LOOH) are formed.  These can react additionally to form later stage end products such as malondialdehyde (MDA) and hydroxynonenal (HNE).  LOOH is sometimes measured to quantify early stage or acute lipid peroxidation while MDA is commonly measured to quantify late stage or chronic lipid peroxidation. More recently, it has been reported that 13-hydroperoxyoctadecanoic acid (13-HPODE), a precursor to 13-hydroxyoctadecanoic acid (13-HODE) can react with proteins to form measurable adducts by covalently binding to specific amino acid residues.  The Hexanoyl-Lysine (HEL) adduct is formed upon oxidative modification of omega-6 fatty acids such as linoleic acid, the predominant polyunsaturated fatty acid (PUFA) in the human diet, and arachidonic acid. HEL may be another useful biomarker for detecting and quantifying the earlier stages of lipid peroxidation.

Catalog: NWK-ISO01
NWLSS™ 8-Isoprostane ELISA Assay
This product is intended for the quantification of 15-isoprostane F2t (also known as 8-epi-PGF2a, 8-iso-PGF2a or more commonly 8-Isoprostane) and can be used with urine, serum or tissue samples following solid phase extraction of the isoprostane-containing fraction. Instructions are also provided for the quantification of total 15-isoprostane F2t following hydrolysis of phospholipids. NOTE: Alternatively, urine samples may be analyzed without solid phase extraction using the NWLSS™ NWK-ISO2 kit.

Catalog: NWK-ISO02
NWLSS™ Urinary 8-Isoprostane Assay
We recommend this assay as a means of quantifying free 15-isoprostane F2t also known as 8-isoprostane, the best characterized isoprostane, in urine, without the need to extract the samples prior to testing. NOTE: Plasma, tissue or cell lysates may be tested using the NWLSS™ NWK-ISO01 assay for isoprostanes. Detailed extraction procedures are included with the product.

Catalog: NWK-LHP01
NWLSS™ Lipid Hydroperoxide Assay
Oxidative damage to lipids (lipid peroxidation) has been found to play an important role in various disease and aging processes. During the early stages of lipid peroxidation, lipid hydroperoxides (LOOH) are formed as a result of fatty acidoxidation by any species sufficiently reactive to abstract hydrogen from a methylene group, to form a carbon-centered radical that reacts with molecular oxygen to form the lipid hydroperoxide. LOOH can be used as a biomarker to detect and quantify early stage lipid peroxidation.

Catalog: NWK-MDA01
NWLSS™ Malondialdehyde Assay
The NWLSS™ Malondialdehyde assay utilizes an improved Thiobarbituric Acid (TBA) based technology, still the most widely published method for testing lipid peroxidation in biological samples. The NWLSS™ NWK-MDA01 assay is designed as a simple, affordable method for testing lipid peroxidation standardized as malondialdehyde (MDA). Unlike other TBA based assays, the NWLSS™ assay utilizes lower heating temperatures, antioxidants to prevent lipid peroxidation artifacts and an improved data reduction method to reduce non-specific TBARS related background interference.

Catalog: NWK-OXLDL01
Oxidized Low Density Lipoprotein ELISA (Ox-LDL-EIA Sandwich Format)
96 well sandwich ELISA for the detection of Oxidized LDL in biological samples. Optimized for human samples however some crossreactivity in rat and pig species has been noted.

Catalog: NWK-OXLDL02
Oxidized Low Density Lipoprotein ELISA (Ox-LDL-EIA Competitive Format)
96 well Competitive ELISA for the detection of Oxidized LDL in biological samples. The competive (NWK-01158) assay requires less sample dilution and yields slightly better recovery values than the sandwich (NWK-01143) ELISA. It is expected to work better in non-human model systems than the Sandwich (NWK-01143) ELISA.

Oxidative/Nitrosative Protein Biomarkers

Catalog: NWK-NTR01
NWLSS™ Nitrotyrosine ELISA
The NWLSS™ is recommended for the detection of nitrosylated proteins in plasma or serum samples. Excellent tool for peroxynitrate related studies and recommended as a biomarker of protein related oxidative/nitrosative stress related augmentation.

Catalog: NWK-PCK01
Protein Carbonyl ELISA
Protein carbonyls are formed by a variety of oxidative mechanisms and can be used as an index of oxidative injury. Recommended as a means to detect general protein oxidation in biological fluids such as plasma, serum, bronchoalveolar lavage, cerebrospinal fluid, cell extracts and other soluble protein samples.

Antioxidant Status Biomarkers
Catalog: NWK-ARC01
NWLSS™ Antioxidant Reductive Capacity (Antioxidant Status) Assay
The NWLSS™ Antioxidant Capacity kit measuring a sample's ability to reduce Cu++ to Cu+ as a means of assessing antioxidant status. The kit is useful as a simple method to quantify generic antioxidant capacity in many types of aqueous samples including serum, plasma, cell culture media and food extracts.

Catalog: NWK-TAC01
NWLSS™ Total Antioxidant Capacity against Peroxyl Radical (TAC-Peroxyl) Assay
The NWLSS™ TAC-Peroxyl Antioxidant Capacity kit is intended for the quantitative measurement of a sample's antioxidant status against peroxyl radical challenge. This product represents a more concise tool allowing investigators to assess a sample's antioxidant capacity against a specific, biologically relevant free radical species. The peroxyl radical is involved in lipid peroxidation and other biologically significant pathways. This product is useful for testing many types of samples including blood plasma or serum, CSF, tissue homogenates, cell lysates, seminal plasma, wine, fruit juices, brewed teas and other botanical extracts. Data generated is in Trolox equivalents similar to the popular ORAC assay.

Endogenous Antioxidant Biomarkers
Catalog: NWK-CAT01
NWLSS™ Catalase Enzyme Activity Assay
Recommended for researchers wishing to assay catalase activity in tissue homogenates, cell lysates & other biological fluids or extracts where catalase enzyme might be present.

Catalog: NWK-CAT02
NWLSS™ Catalase Enzyme Activity Assay
650nm (colorimetric in visible range) method with sensitivity of 10 U Catalase/mL sample added to the reaction. Recommended for researchers wishing to assay catalase activity in tissue homogenates, cell lysates & other biological fluids or extracts where catalase enzyme might be present.

Catalog: NWK-GPX01
NWLSS™ Glutathione Peroxidase Assay
The NWLSS™ Glutathione Peroxidase Assay kit is a kinetic, rate based assay for quantification of glutathione peroxidase (GPx) in a variety of samples including blood products, tissues and cultured cells.

Catalog: NWK-GR01
NWLSS™ Glutathione Reductase (Microplate) Assay
The NWLSS™ Glutathione Reductase Assay kit is a kinetic, rate based assay for quantification of glutathione reductase (GR) in a variety of samples including blood cells, tissues and cultured cells.

Catalog: NWK-GSH01
NWLSS™ Glutathione Assay
The NWLSS™ Glutathione Assay kit is a kinetic, rate based assay for quantification of total GSH (reduced and oxidized) in a variety of samples including blood, plasma, tissues and cultured cells. The high sensitivity method provides a limit of detection down to 0.25 mM, suitable for measurement of oxidized GSH (GSSG) after scavenging of reduced GSH.

Catalog: NWK-SOD01
NWLSS™ Human Cu/ZnSOD ELISA
The NWLSS™ Cu/ZnSOD ELISA is intended for the quantitative detection of human Cu/ZnSOD in human plasma, serum cell culture supernatants, erythrocyte (RBC) or other cell lysates and other biological fluids where SOD may be present.

Catalog: NWK-SOD02
NWLSS™ Superoxide Dismutase Enzyme Activity Assay
Recommended for researchers wishing to assay SOD activity in tissue homogenates, cell lysates & other biological fluids or extracts where forms of SOD might be present. Suitable for quantifying MnSOD activity when KCN is added to assay buffer to inhibit Cu/ZnSOD activity.

Inflammatory Biomarkers

Catalog: NWK-HYP01
NWLSS™ Hydrogen Peroxide Assay
This kit is designed to test for hydrogen peroxide content of aqueous biological samples.

Catalog: NWK-MPO02
NWLSS™ Human Myeloperoxidase (MPO) ELISA
MPO has been implicated as playing a role in the oxidation of low density lipoprotein (LDL) and the advancement of cardiovascular disease. This kit has been optimized for measurement of MPO "expression" in human plasma and tissue samples. Cross reactivity in rodent model systems has not been tested. The activity of MPO enzyme from any species can be tested using product number NWK-MPO03.

Catalog: NWK-MPO03
NWLSS™ Myeloperoxidase Activity Assay
This kit is designed to test for myeloperoxidase enzyme activity in biological samples. Unlike the NWK-MPO02 assay, this assay is suitable for use with samples from any species