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Northwest
Assay Kits


Antioxidant Status

Catalog: NWK-TAC01
NWLSS™ Total Antioxidant Capacity against Peroxyl Radical (TAC-Peroxyl) Assay
The NWLSS™ TAC-Peroxyl Antioxidant Capacity kit is intended for the quantitative measurement of a sample's antioxidant status against peroxyl radical challenge. This product represents a more concise tool allowing investigators to assess a sample's antioxidant capacity against a specific, biologically relevant free radical species. The peroxyl radical is involved in lipid peroxidation and other biologically significant pathways. This product is useful for testing many types of samples including blood plasma or serum, CSF, tissue homogenates, cell lysates, seminal plasma, wine, fruit juices, brewed teas and other botanical extracts. Data generated is in Trolox equivalents similar to the popular ORAC assay.

Catalog: NWK-ARC01
NWLSS™ Antioxidant Reductive Capacity (Antioxidant Status) Assay
The NWLSS™ Antioxidant Capacity kit measuring a sample's ability to reduce Cu++ to Cu+ as a means of assessing antioxidant status. The kit is useful as a simple method to quantify generic antioxidant capacity in many types of aqueous samples including serum, plasma, cell culture media and food extracts.

Endogenous Antioxidants

Catalog: NWK-CAT01
NWLSS™ Catalase Enzyme Activity...UV Assay
240nm (UV) method with sensitivity of 0.3 U catalase/mL reaction mixture. Recommended for researchers wishing to assay catalase activity in tissue homogenates, cell lysates & other biological fluids or extracts where catalase enzyme might be present. Recommended for model systems where higher sensitivity may be required.

Catalog: NWK-CAT02
NWLSS™ Catalase Enzyme Activity...Colorimetric Assay
650nm (colorimetric in visible range) method with sensitivity of 10 U Catalase/mL sample added to the reaction. Recommended for researchers wishing to assay catalase activity in tissue homogenates, cell lysates & other biological fluids or extracts where catalase enzyme might be present.

Catalog: NWK-GPX01
NWLSS™ Glutathione Peroxidase Assay
The NWLSS™ Glutathione Peroxidase Assay kit is a kinetic, rate based assay for quantification of glutathione peroxidase (GPx) in a variety of samples including blood products, tissues and cultured cells.

Catalog: NWK-GR01
NWLSS™ Glutathione Reductase (Microplate) Assay
The NWLSS™ Glutathione Reductase Assay kit is a kinetic, rate based assay for quantification of glutathione reductase (GR) in a variety of samples including blood cells, tissues and cultured cells.

Catalog: NWK-GSH01
NWLSS™ Glutathione Assay
The NWLSS™ Glutathione Assay kit is a kinetic, rate based assay for quantification of total GSH (reduced and oxidized) in a variety of samples including blood, plasma, tissues and cultured cells. The high sensitivity method provides a limit of detection down to 0.25 mM, suitable for measurement of oxidized GSH (GSSG) after scavenging of reduced GSH.

Catalog: NWK-SOD01
NWLSS™ Human Cu/ZnSOD ELISA
The NWLSS™ Cu/ZnSOD ELISA is intended for the quantitative detection of human Cu/ZnSOD in human plasma, serum cell culture supernatants, erythrocyte (RBC) or other cell lysates and other biological fluids where SOD may be present.

Catalog: NWK-SOD02
NWLSS™ Superoxide Dismutase Enzyme Activity Assay
Recommended for researchers wishing to assay SOD activity in tissue homogenates, cell lysates & other biological fluids or extracts where forms of SOD might be present. Suitable for quantifying MnSOD activity when KCN is added to assay buffer to inhibit Cu/ZnSOD activity.

16420 S.E. McGillivray, Suite 103, PBM 106, Vancouver, WA 98683
Phone: (360) 449-3091 or (888) 449-3091, Fax: (360) 449-3092
E-mail: nwinfo@nwlifescience.com

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