Monoclonal Anti-8-Hydroxydeoxyguanosine...Clone N45.1
100 µg (lyophilized)...Catalog: NWA-MOG100, View Datasheet
20 µg (lyophilized)...Catalog: NWA-MOG020, View Datasheet
8-hydroxy-2'-deoxyguanosine (8-OHdG) is formed when DNA is oxidatively modified by reactive oxygen species (ROS). 8-OHdG is one of the most sensitive biomarkers for oxidative stress and can be detected in various biological sample types.
Catalog: NWA-8OHdG
Monoclonal Anti-8-Hydroxydeoxyguanosine...Clone 15A3...Liquid
100 µg (liquid)...Catalog: NWA-8OHDG, View Datasheet
8-hydroxy-2'-deoxyguanosine (8-OHdG) is formed when DNA is oxidatively modified by reactive oxygen species (ROS). 8-OHdG is one of the most sensitive biomarkers for oxidative stress and can be detected in various biological sample types.
Lipid Peroxidation Biomarkers
Monoclonal Anti-Hydroxynonenal (4-HNE)...Clone HNEJ-2
100 µg (lyophilized)...Catalog: NWA-HNE100, View Datasheet
20 µg (lyophilized)...Catalog: NWA-HNE020, View Datasheet
This antibody shows almost negligible reactivity with proteins that were treated
with other aldehydes, such as 2nonenal,
2hexenal,
1hexanal,
4hydroxy2hexenal,
formaldehyde, or glutaraldehyde.
Monoclonal Anti-Malondialdehyde...Clone 1F83
30 µg (liquid)...Catalog: NWA-MDA030, View Datasheet
Malondialdehyde (MDA) is one of the major aldehydes
derived from lipid peroxidation. MDA is highly reactive
aldehyde and reacts with lysine residue in protein. The
reaction with MDA and lysine residue leads to the
formation of numerous numbers of adducts, such as
dihydropyridinelysine
(DHPlysine)
type derivative.
This monoclonal antibody is specific for the
MDAmodified
protein, especially DHPlysine
type
derivative.
Monoclonal Anti-Crotonaldehyde
30 µg (liquid)...Catalog: NWA-CRA030, View Datasheet
Crotonaldehyde (CRA) is a representative
carcinogenic aldehyde formed endogenously
through lipid peroxidation. CRA is highly reactive
aldehyde and reacts with lysine residue in protein.
The reaction with CRA and lysine residue leads to
the formation of numerous numbers of adducts.
This antibody is specific for the CRAmodified
protein.
Monoclonal Anti-Hexanoyl-Lysine...Clone 5H4
20 µg (lyophilized)...Catalog: NWA-HEL020, View Datasheet
Oxidative damage to lipids (lipid peroxidation) has been found to play an important role in various disease and aging processes.
During early stages of lipid peroxidation, lipid hydroperoxides (LOOH) are formed.
These can react additionally to form later stage end products such as malondialdehyde (MDA) and hydroxynonenal (HNE).
LOOH is sometimes measured to quantify early stage or acute lipid peroxidation while MDA is commonly measured to quantify late stage or chronic lipid peroxidation.
More recently, it has been reported that 13-hydroperoxyoctadecanoic acid (13-HPODE), a precursor to 13-hydroxyoctadecanoic acid (13-HODE) can react with proteins to form measurable adducts by covalently binding to specific amino acid residues.
The Hexanoyl-Lysine (HEL) adduct is formed upon oxidative modification of omega-6 fatty acids such as linoleic acid, the predominant polyunsaturated fatty acid (PUFA) in the human diet, and arachidonic acid.
HEL may be another useful biomarker for detecting and quantifying the earlier stages of lipid peroxidation.
Monoclonal Anti-Acrolein...Clone F56
20 µg (lyophilized)...Catalog: NWA-ACR020, View Datasheet
Acrolein(CH2=CHCHO; acrolein, ACR) is a chemical substance formed not only by combustion of petroleum, charcoal, lumber and plastic but also by cigarette smoke, exhaust gas, heating of oils and fats and is known as a strong cytotoxic substance. In recent years it was revealed by Uchida et al., of Nagoya University that ACR can also be produced by hyperoxidation of lipids and its presence as a secondary product resulting from lipid peroxidation in-vivo was identified. ACR's effect on biological systems has now become a focus of attention.
The addition of Acrolein to protein lysine residues results in the formation of formyl-dehydro-piperidino-lysine (FDPLys) adducts.
Monoclonal Anti-Methylglyoxal...Clone 3C
30 µg (liquid)...Catalog: NWA-MGL030, View Datasheet
Methylglyoxal (MG), an endogenous metabolite that increases in diabetes and is a common
intermediate in the Maillard glycation reaction. MG reacts with proteins and forms advanced
glycation end products (AGE’s). MG reacts with residues in proteins and forms numerous
adducts,such as argpyrimidine.
Oxidized Protein Biomarkers
Monoclonal Anti-Di Tyrosine (DT)...Clone 1C3
20 µg (lyophilized)...Catalog: NWA-DIY020, View Datasheet
This antibody binds to dityrosine, 3-(p-hydroxyphenyl) propionic acid dimer,
dityrosine-conjugated BSA, Dityrosine in proteins and peptides.
Monoclonal Anti-Di bromo tyrosine...Clone 3A5
20 µg (lyophilized)...Catalog: NWA-DBY020, View Datasheet
Myeloperoxidase and eosinophil peroxidase are known to
catalyze formation of hypochlorous acid (HOCl) and hypobromous acid (HOBr).
These reactive intermediates react with proteins, and are known to form tyrosine
halogenation such as dibromotyrosine (DiBrY).
This monoclonal antibody is specific for 3,5-DiBrY, and is suitable for
immunohistochemical analysis of oxidative stress.
